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Related Experiment Videos

Highly sensitive optical chip immunoassays in human serum.

B H Schneider1, E L Dickinson, M D Vach

  • 1Photonic Sensor, Atlanta, GA 30308, USA. schneider@photonicsensor.com

Biosensors & Bioelectronics
|May 29, 2000
PubMed
Summary

This study presents a novel one-step assay for rapid detection of human chorionic gonadotropin (hCG) in serum. By minimizing non-specific binding (NSB), the assay achieves high sensitivity for biomolecular detection in complex fluids.

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Optical chip immunoassay for hCG in human whole blood.

Biosensors & bioelectronics·2001

Area of Science:

  • Biomolecular detection
  • Optical sensing technologies
  • Nanoparticle-enhanced assays

Background:

  • Refractometric optical sensors can quantify various biomolecules, achieving high sensitivity with nanoparticle amplification.
  • Detecting analytes in complex biological fluids like serum using a one-step procedure remains challenging due to non-specific binding (NSB).

Purpose of the Study:

  • To develop a rapid, one-step assay for detecting human chorionic gonadotropin (hCG) in human serum.
  • To mitigate interference from non-specific binding (NSB) in complex biological fluids using an optical chip based on the Hartman interferometer.

Main Methods:

  • Utilized a Hartman interferometer with separate reference and specific sensing regions to control NSB.
  • Employed dry storage of functionalized optical chips followed by rehydration in serum.

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  • Optimized surface chemistry using a biotin-poly(ethylene glycol) (PEG) blocking agent.
  • Developed a one-step sandwich assay incorporating gold nanoparticles for signal amplification.

    • Main Results:

    • Reduced background drift in serum by an order of magnitude using an exposed reference compared to a blind reference.
    • Achieved an additional 70% reduction in signal drift by employing a biotin-PEG blocking agent.
    • Established a one-step assay for hCG in human serum with a detection limit of 0.1 ng/ml.
    • Demonstrated a rapid assay duration of 35 minutes.

    Conclusions:

    • The developed Hartman interferometer-based optical chip effectively controls NSB in complex serum samples.
    • The optimized surface chemistry and assay format enable sensitive and rapid detection of hCG in a one-step procedure.
    • This approach holds promise for sensitive, rapid, and one-step biomolecular detection in complex biological fluids.