Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Detection in capillary electrophoresis.

K Swinney1, D J Bornhop

  • 1Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, USA.

Electrophoresis
|May 29, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Imaging and quantitation of a tissue-selective lanthanide chelate using an endoscopic fluorometer.

Journal of biomedical optics·2012
Same author

Recent advances in receptor-targeted fluorescent probes for in vivo cancer imaging.

Current medicinal chemistry·2012
Same author

Microvolume index of refraction determinations by interferometric backscatter.

Applied optics·2010
Same author

Role of the antenna in tissue selective probes built of lanthanide-organic chelates.

The journal of physical chemistry. A·2008
Same author

Nanoliter-scale non-invasive flow-rate quantification using micro-interferometric back-scatter and phase detection.

Fresenius' journal of analytical chemistry·2001
Same author

Noninvasive picoliter volume thermometry based on backscatter interferometry.

Electrophoresis·2001
Same journal

Kinship Inferences for Second-Degree Relatives With a Combination of STRs and Microhaplotypes.

Electrophoresis·2026
Same journal

Optimisation of Electrokinetic Extraction System: Colourimetric Determination of Copper (II) in Sand Using Polymer Inclusion Membrane.

Electrophoresis·2026
Same journal

Novel Phloroglucinol Derivatives as Neuraminidase Inhibitors Identified From Humulus lupulus L. Extract by At-Line Nanofractionation Platform.

Electrophoresis·2026
Same journal

Protein-Based High-Performance Liquid Chromatography and Cyclodextrin-Capillary Electrokinetic Chromatography for the Chiral Separation of Azoles.

Electrophoresis·2026
Same journal

Dynamics of Heparin Translocations Through Solid-State Nanopores.

Electrophoresis·2026
Same journal

Production of Protein Hydrolysates and Bioactive Peptides From Lablab purpureus and Macrotyloma uniflorum via Optimized Extraction and Proteolysis Protocols.

Electrophoresis·2026
See all related articles

This review covers four main capillary electrophoresis (CE) detection methods: fluorescence, absorbance, electrochemical, and refractive index. The best method depends on the specific application due to varying detection limits and complexity.

Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Spectroscopy

Background:

  • Capillary electrophoresis (CE) is a powerful separation technique.
  • Effective detection is crucial for CE analysis.
  • Multiple online detection modalities exist for CE.

Purpose of the Study:

  • To review the four major online detection modalities for capillary electrophoresis.
  • To compare the advantages and limitations of each detection method in a CE context.
  • To provide a perspective on the applicability of CE detection techniques.

Main Methods:

  • Review of literature on fluorescence detection in CE.
  • Analysis of absorbance (conventional and nonconventional) detection in CE.
  • Evaluation of electrochemical detection in CE.

Related Experiment Videos

  • Assessment of refractive index detection in CE.

    • Main Results:

    • Each CE detection method (fluorescence, absorbance, electrochemical, refractive index) exhibits unique strengths and weaknesses.
    • Detection limits vary significantly across methods, from single molecule sensitivity to 10(-5) M.
    • The complexity of detection schemes differs substantially among the modalities.

    Conclusions:

    • The choice of CE detection methodology should be guided by the specific analytical requirements of the application.
    • Understanding the distinct advantages and limitations of each detection technique is essential for optimal method development in CE.
    • CE offers a versatile platform for chemical analysis when paired with appropriate detection strategies.