Jove
Visualize
Contact Us

Related Experiment Videos

Nanoliter-scale non-invasive flow-rate quantification using micro-interferometric back-scatter and phase detection.

D A Markov1, D J Bornhop

  • 1Department of Chemistry and Biochemistry, Texas Tech University, Lubbock 79409-1061, USA.

Fresenius' Journal of Analytical Chemistry
|October 27, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[The operational characteristics of modes of differential diagnostic of benign and malignant neoplasms of skeletal system].

Klinicheskaia laboratornaia diagnostika·2014
Same author

Imaging and quantitation of a tissue-selective lanthanide chelate using an endoscopic fluorometer.

Journal of biomedical optics·2012
Same author

Recent advances in receptor-targeted fluorescent probes for in vivo cancer imaging.

Current medicinal chemistry·2012
Same author

Graphene transistor as a probe for streaming potential.

Nano letters·2012
Same author

Microvolume index of refraction determinations by interferometric backscatter.

Applied optics·2010
Same author

Mirrored pyramidal wells for simultaneous multiple vantage point microscopy.

Journal of microscopy·2008
Same journal

Bioluminescence, chemiluminescence.

Fresenius' journal of analytical chemistry·2020
Same journal

Symposium 3: Non-enzymatic biocatalysts in nature and biotechnology.

Fresenius' journal of analytical chemistry·2020
Same journal

Direct determination of boron and zirconium in ceramic materials by flame atomic absorption spectrometry after alkali sintering and fusion.

Fresenius' journal of analytical chemistry·2002
Same journal

Derivative hydride generation atomic absorption spectrometry and determination of lead traces in waters.

Fresenius' journal of analytical chemistry·2002
Same journal

Investigation of contemporary gilded forgeries of ancient coins.

Fresenius' journal of analytical chemistry·2002
Same journal

Chemical modifiers for direct determination of cobalt in coal combustion residues by ultrasonic slurry-sampling-ETAAS.

Fresenius' journal of analytical chemistry·2002
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Researchers developed a non-invasive method to measure fluid velocity in tiny capillaries. This technique uses laser heating and a special detector to accurately quantify flow rates in microfluidic devices.

Area of Science:

  • Microfluidics
  • Analytical Chemistry
  • Optical Sensing

Background:

  • Miniaturization of fluid-handling systems, especially microfluidic devices, enhances chemical and biochemical analyses.
  • Accurate, non-invasive measurement of flow velocity in nano- and picoliter volumes remains challenging.

Purpose of the Study:

  • To present a simple, non-invasive method for detecting and measuring linear flow velocity in fluid-filled capillaries.
  • To enable precise flow rate quantification in microscale fluidic systems.

Main Methods:

  • Utilized local heating of a small fluid volume with an infrared laser diode.
  • Employed a micro-interferometric back-scatter detector (MIBD) downstream to detect thermally induced refractive index changes.
  • Calculated fluid velocity by analyzing the phase difference between the heating function and MIBD output in the Fourier domain.

Related Experiment Videos

Main Results:

  • Quantified flow rates between 1 and 10 µL/min within a 40 nL probe volume.
  • Achieved a 3-sigma detection limit of 42.8 nL/min for flow velocity measurements.
  • Demonstrated a non-invasive approach for microscale fluid dynamics analysis.

Conclusions:

  • The presented method offers a viable solution for accurate flow velocity measurement in microfluidic channels.
  • This technique supports the advancement of miniaturized analytical systems requiring precise fluid control.
  • The non-invasive nature and sensitivity make it suitable for various microfluidic applications.