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In Vitro ELISA Test to Evaluate Rabies Vaccine Potency
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In Vitro ELISA Test to Evaluate Rabies Vaccine Potency

Published on: May 11, 2020

Evaluation of a blocking ELISA using a urease conjugate for the detection of antibodies to pseudorabies virus

M G Echeverría1, E O Nosetto, M E Etcheverrigaray

  • 1Area of Virology, Faculty of Veterinary Sciences, National University of La Plata, Argentina.

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
|May 29, 2000
PubMed

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.