Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nitroazole universal bases in peptide nucleic acids.

H Challa1, M L Styers, S A Woski

  • 1Department of Chemistry and Coalition for Biomolecular Products, University of Alabama, Tuscaloosa 35487-0336, USA.

Organic Letters
|June 3, 2000
PubMed
Summary

Novel nucleobase analogues, 3-nitropyrrole and 5-nitroindole, were synthesized for peptide nucleic acid (PNA) oligomers. These PNA analogues show consistent hybridization with DNA, expanding PNA applications for imprecise sequence data.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

BLOC-1 complex deficiency alters the targeting of adaptor protein complex-3 cargoes.

Molecular biology of the cell·2006
Same author

Incorporation of positively charged deoxynucleic S-methylthiourea linkages into oligodeoxyribonucleotides.

Bioorganic & medicinal chemistry letters·2001
Same author

Disiloxane-protected 2-deoxyribonolactone as an efficient precursor to 1,2-dideoxy-1-beta-aryl-D-ribofuranoses.

Organic letters·2000
Same author

The binding of trivalent chromium to low-molecular-weight chromium-binding substance (LMWCr) and the transfer of chromium from transferrin and chromium picolinate to LMWCr.

Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry·2000
Same author

Design of modified oligodeoxyribonucleotide probes to detect telomere repeat sequences in FISH assays.

Nucleic acids research·1999
Same author

The nutritional supplement chromium(III) tris(picolinate) cleaves DNA.

Chemical research in toxicology·1999

Area of Science:

  • Biochemistry
  • Organic Chemistry
  • Molecular Biology

Background:

  • Peptide nucleic acids (PNAs) are DNA mimics with unique binding properties.
  • Developing PNA analogues with versatile hybridization capabilities is crucial for expanding their applications.
  • Ambiguous nucleobase analogues offer potential for degenerate sequencing applications.

Purpose of the Study:

  • To synthesize novel PNA oligomers incorporating 3-nitropyrrole and 5-nitroindole analogues.
  • To evaluate the hybridization properties of these modified PNAs with complementary oligodeoxynucleotides.
  • To assess the potential of these analogues for applications requiring degenerate sequencing.

Main Methods:

  • Chemical synthesis of PNA oligomers containing 3-nitropyrrole and 5-nitroindole.

Related Experiment Videos

  • Thermal denaturation (Tm) experiments to assess hybridization affinity.
  • Comparison of Tm values against all four standard nucleosides (A, T, C, G).

    • Main Results:

    • Successful synthesis of PNA oligomers with 3-nitropyrrole and 5-nitroindole analogues.
    • Both novel analogues demonstrated minimal variation in hybridization Tm (< or = 1.5 degrees C) against all complementary bases.
    • The Tm stability indicates broad compatibility with standard DNA sequences.

    Conclusions:

    • 3-nitropyrrole and 5-nitroindole are viable ambiguous nucleobase analogues for PNA synthesis.
    • These analogues maintain stable hybridization, suggesting utility in degenerate PNA applications.
    • The incorporation of degenerate sites expands PNA's applicability in areas with unknown or variable sequences.