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Updated: Aug 12, 2026

Myosin-Specific Adaptations of In vitro Fluorescence Microscopy-Based Motility Assays
Published on: February 4, 2021
Single-motor mechanics and models of the myosin motor
T Yanagida1, S Esaki, A H Iwane
1Exploratory Research for Advanced Technology, Japan Science and Technology Corporation, Mino, Osaka. yanagida@physl.med.osaka-u.ac.jp
Abstract:
Recent progress in single-molecule detection techniques is remarkable. These techniques have allowed the accurate determination of myosin-head-induced displacements and how mechanical cycles are coupled to ATP hydrolysis, by measuring individual mechanical events and chemical events of actomyosin directly at the single-molecule level. Here we review our recent work in which we have made detailed measurements of myosin step size and mechanochemical coupling, and propose a model of the myosin motor.
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