Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Two lineage boundaries coordinate vertebrate apical ectodermal ridge formation.

R A Kimmel1, D H Turnbull, V Blanquet

  • 1Department of Cell Biology, New York University School of Medicine, New York, New York 10016 USA.

Genes & Development
|June 3, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genome Wide Conditional Mouse Knockout Resources.

Drug discovery today. Disease models·2024
Same author

Somatic gene editing ameliorates skeletal and cardiac muscle failure in pig and human models of Duchenne muscular dystrophy.

Nature medicine·2020
Same author

Buckling without Bending: A New Paradigm in Morphogenesis.

Physical review. X·2019
Same author

Cross-disorder risk gene CACNA1C differentially modulates susceptibility to psychiatric disorders during development and adulthood.

Molecular psychiatry·2017
Same author

The pathogenic LRRK2 R1441C mutation induces specific deficits modeling the prodromal phase of Parkinson's disease in the mouse.

Neurobiology of disease·2017
Same author

A paternal methyl donor-rich diet altered cognitive and neural functions in offspring mice.

Molecular psychiatry·2017
Same journal

Corrigendum: Inhibition of Myc family proteins eradicates KRas-driven lung cancer in mice.

Genes & development·2026
Same journal

A new perspective on ATR's role in translesion synthesis.

Genes & development·2026
Same journal

Mechanisms coordinating exit from the stem cell state in mammals.

Genes & development·2026
Same journal

Evolutionarily conserved spliceosome-exosome pathway in nuclear mRNA surveillance.

Genes & development·2026
Same journal

CDK1 and CEP97 cooperatively control centriole length to orchestrate ciliogenesis and developmental patterning.

Genes & development·2026
Same journal

Coupling of translesion synthesis with the replisome stabilized at stalled replication forks by ATR.

Genes & development·2026
See all related articles

The apical ectodermal ridge (AER) controls vertebrate limb bud outgrowth. Mouse studies reveal two ectodermal lineage boundaries crucial for AER formation and positioning, regulated by the En1 gene.

Area of Science:

  • Developmental Biology
  • Molecular Genetics
  • Embryology

Background:

  • The apical ectodermal ridge (AER) is essential for vertebrate limb bud proximal-distal outgrowth.
  • AER formation occurs at a fixed position along the embryonic dorsal-ventral (D/V) axis.

Purpose of the Study:

  • Investigate the genetic and cellular mechanisms regulating AER formation in mice.
  • Identify lineage boundaries and signaling pathways involved in AER development.

Main Methods:

  • Cre/loxP-based fate mapping in mice.
  • Novel retroviral cell-labeling techniques.
  • Inducible Cre-mediated labeling of AER precursors.
  • Transgenic mouse models with altered En1 expression.

Related Experiment Videos

Main Results:

  • Identified two distinct ectodermal lineage boundaries prior to limb bud outgrowth.
  • Demonstrated that not all AER gene-expressing cells form the AER, requiring signaling for maintenance.
  • Showed En1 misexpression alters AER formation and represses Wnt7a expression dorsally.
  • Fate mapping confirmed En1's role in positioning and maintaining lineage borders.

Conclusions:

  • En1 is critical for establishing and maintaining AER lineage boundaries.
  • Signaling regulated by En1 acts across the D/V border, influencing AER development.
  • Mouse AER formation involves distinct lineage segregation and signaling maintenance mechanisms.