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Related Experiment Videos

The disruption of adherens junctions is associated with a decrease of E-cadherin phosphorylation by protein kinase

M Serres1, O Filhol, H Lickert

  • 1INSERM U 346, Hôpital E. Herriot, Lyon Cedex 03, 69437, France. serres@lyon151.inserm.fr

Experimental Cell Research
|June 6, 2000
PubMed
Summary

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E-cadherin phosphorylation by CK2 is crucial for maintaining skin cell adhesion. Decreased phosphorylation disrupts cell junctions, impacting epidermal cohesion and potentially contributing to cancer development.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Down-regulation of E-cadherin is a hallmark of carcinogenesis.
  • Posttranscriptional modifications, like phosphorylation, regulate E-cadherin function.
  • E-cadherin mediates cell-cell adhesion, essential for tissue integrity.

Purpose of the Study:

  • To investigate the role of E-cadherin phosphorylation in keratinocyte cell-cell adhesion.
  • To identify the kinase responsible for E-cadherin phosphorylation.
  • To elucidate the mechanism by which phosphorylation affects E-cadherin localization and function.

Main Methods:

  • Treatment of keratinocytes with okadaic acid to induce dephosphorylation.
  • Fractionation of cell lysates to analyze Triton-soluble and insoluble components.

Related Experiment Videos

  • Western blotting and co-immunoprecipitation to detect E-cadherin and CK2.
  • Use of specific CK2 inhibitors (heparin, DRB) to assess kinase activity.

    • Main Results:

    • Okadaic acid treatment decreased E-cadherin phosphorylation, leading to membrane-to-cytoplasm shift and impaired cell aggregation.
    • Reduced phosphorylation was observed in the Triton-soluble fraction, indicating a role in cell-cell interactions.
    • Casein kinase 2 (CK2) was identified as the primary kinase phosphorylating E-cadherin.
    • CK2 inhibition or impaired CK2 phosphorylation capacity disrupted E-cadherin function.

    Conclusions:

    • E-cadherin phosphorylation by CK2 is vital for maintaining epidermal cohesion.
    • Dysregulation of this phosphorylation process can lead to loss of cell-cell adhesion.
    • CK2's role in E-cadherin phosphorylation is critical for skin integrity and may be relevant in carcinogenesis.