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Homogeneous Cell- and Bead-Based Assays for High Throughput Screening Using Fluorometric Microvolume Assay

Miraglia1, Swartzman, Mellentin-Michelotti

  • 1PE Biosystems, Foster City, CA.

Journal of Biomolecular Screening
|June 6, 2000
PubMed
Summary
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Fluorometric microvolume assay technology (FMAT) offers a nonradioactive, miniaturized solution for high throughput drug screening. This fluorescence-based platform enables homogeneous assays, streamlining the drug discovery process.

Area of Science:

  • Biochemistry
  • Assay Development
  • Drug Discovery

Background:

  • High throughput screening (HTS) is essential for modern drug discovery.
  • Miniaturization and nonradioactive formats are key requirements for HTS assays.
  • Homogeneous assays reduce automation needs, improving efficiency.

Purpose of the Study:

  • To introduce Fluorometric Microvolume Assay Technology (FMAT) as a novel platform for HTS.
  • To demonstrate the versatility and applicability of FMAT in various assay formats.
  • To highlight the advantages of FMAT for drug screening.

Main Methods:

  • FMAT utilizes a fluorescence-based, laser scanning system to image microwell plates.
  • The technology detects localized fluorescence from cells or beads at the bottom of wells.

Related Experiment Videos

  • Data processing ignores unbound fluorophore, enabling homogeneous assay development.

    • Main Results:

    • FMAT is compatible with various assay types, including ligand binding, immunofluorescence, apoptosis, cytotoxicity, and bead-based assays.
    • The system is plate format-independent and integrates with robotic automation for unattended operation.
    • Localized fluorescence detection allows for sensitive and specific assay results.

    Conclusions:

    • FMAT provides a robust, nonradioactive, and miniaturized solution for HTS.
    • The technology supports a wide range of homogeneous assays, enhancing drug discovery efficiency.
    • FMAT is a valuable tool for developing and implementing advanced drug screening platforms.