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Related Experiment Videos

Rapid identification of Staphylococcus epidermidis.

M Wieser, H J Busse

    International Journal of Systematic and Evolutionary Microbiology
    |June 8, 2000
    PubMed
    Summary

    Repetitive-sequence PCR, specifically enterobacterial repetitive intergenic consensus (ERIC) PCR and BOX-PCR, effectively identified Staphylococcus epidermidis. These molecular methods offer a reliable alternative for species identification when biochemical tests are inconclusive.

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    Area of Science:

    • Microbiology
    • Molecular Biology
    • Bacterial Identification

    Background:

    • Bacterial isolates from a museum environment were identified as Staphylococcus species based on fatty acid profiles.
    • Coagulase-negative staphylococci present challenges for species-level identification due to similar fatty acid compositions.
    • Initial biochemical testing suggested the isolates were Staphylococcus epidermidis with high probability.

    Purpose of the Study:

    • To genotypically characterize Staphylococcus epidermidis isolates.
    • To evaluate the utility of repetitive-sequence PCR (ERIC, BOX, REP) for differentiating Staphylococcus species, particularly Staphylococcus epidermidis.
    • To establish reliable molecular identification methods for coagulase-negative staphylococci.

    Main Methods:

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  • Fatty acid profiling and Staph ID 32 API system for initial identification.
  • Genotypic characterization using repetitive-sequence PCR: enterobacterial repetitive intergenic consensus (ERIC) PCR, BOX-PCR, and repetitive extragenic palindromic unit sequence (REP) PCR.
  • Analysis of Staphylococcus epidermidis strains from various sources and type strains of related staphylococci.

    • Main Results:

    • ERIC-PCR and BOX-PCR generated species-specific banding patterns for all Staphylococcus epidermidis strains.
    • Distinct banding patterns were observed for all staphylococcal reference strains, differentiating them from Staphylococcus epidermidis.
    • REP-PCR did not yield species-specific banding patterns.

    Conclusions:

    • ERIC-PCR and BOX-PCR are highly effective tools for the specific identification of Staphylococcus epidermidis.
    • These molecular techniques overcome the limitations of fatty acid analysis and biochemical tests for coagulase-negative staphylococci identification.
    • Repetitive-sequence PCR offers a robust genotypic approach for bacterial species differentiation.