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Allergic Drug Reactions

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Allergic reactions related to drugs are hypersensitivity responses driven by the immune system and bear no connection to the drug's therapeutic action. While drugs in isolation do not trigger an immune response, they can interact with endogenous proteins to form antigens. These antigens stimulate lymphocytes to produce antibodies. IgE-type antibodies attach themselves to mast cells. Upon subsequent exposure to the same stimulus, the antigen-antibody interaction is initiated, unleashing numerous...

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Acute Inflammation I: Inflammatory Response

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Related Experiment Video

Updated: Jul 1, 2026

Absorption of Nasal and Bronchial Fluids: Precision Sampling of the Human Respiratory Mucosa and Laboratory Processing of Samples

Absorption of Nasal and Bronchial Fluids: Precision Sampling of the Human Respiratory Mucosa and Laboratory Processing of Samples

Published on: January 21, 2018

Temporal patterns of mediator release during developing cutaneous late-phase reactions.

B Zweiman¹, C Von Allmen

¹Department of Medicine, Division of Allergy and Immunology, University of Pennsylvania School of Medicine, Philadelphia, PA, USA.

Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology

|June 10, 2000

Summary

Cutaneous late phase reactions involve rapid inflammatory mediator release and immune cell activation. Histamine, lactoferrin, and eosinophil cationic protein are released early, contributing to skin inflammation.

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Humanized Mediator Release Assay as a Read-Out for Allergen Potency

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Area of Science:

Immunology
Dermatology
Allergy Research

Background:

Cutaneous late phase reactions (LPR) involve inflammatory mediators.
The precise timing of mediator release during LPR development remains unclear.

Purpose of the Study:

To chronologically map mediator release during LPR.
To correlate mediator release with inflammatory cell responses and gross LPR development.

Main Methods:

Utilized skin chambers for hourly sample collection following allergen challenge.
Analyzed exuded leukocytes for activation and mediator content.

Main Results:

Histamine release peaked within the first hour post-allergen challenge.
Neutrophil-derived lactoferrin and eosinophil cationic protein release followed, associated with IL-8.
A higher percentage of activated leukocytes was observed in allergen-challenged sites.

Conclusions:

Skin inflammatory responses, including leukocyte activation and mediator release, commence shortly after initial mast cell activation in IgE-mediated reactions.
These findings elucidate the temporal dynamics of LPR development.