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Related Experiment Videos

Rox1 mediated repression. Oxygen dependent repression in yeast.

A J Kastaniotis1, R S Zitomer

  • 1Dept. Biol. Sci., University at Albany, SUNY, USA.

Advances in Experimental Medicine and Biology
|June 13, 2000
PubMed
Summary

Oxygen levels in Saccharomyces cerevisiae are sensed via heme biosynthesis, regulating gene transcription. Heme

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Roles of transcription factor Mot3 and chromatin in repression of the hypoxic gene ANB1 in yeast.

Molecular and cellular biology·2000

Area of Science:

  • Molecular Biology
  • Yeast Genetics
  • Gene Regulation

Background:

  • Facultative anaerobes like Saccharomyces cerevisiae possess oxygen-regulated genes.
  • Oxygen presence is primarily sensed through heme biosynthesis.
  • Heme influences gene transcription, inducing oxygen-dependent genes and repressing hypoxic genes.

Purpose of the Study:

  • To elucidate the mechanism of oxygen sensing and gene regulation in Saccharomyces cerevisiae.
  • To identify the key proteins involved in the repression of hypoxic genes.

Main Methods:

  • Analysis of gene transcription in response to oxygen levels.
  • Investigating the role of heme biosynthesis in oxygen sensing.
  • Studying the function of Rox1 and Ssn6/Tup1 complex in gene repression.
  • Examining the impact of Mot3 on Rox1-mediated repression.

Main Results:

  • Heme biosynthesis acts as the oxygen sensor in Saccharomyces cerevisiae.
  • Heme induces oxygen-dependent genes and represses hypoxic genes.
  • Rox1 protein, along with the Ssn6/Tup1 complex, mediates the repression of hypoxic genes.
  • Differential repression of hypoxic genes is influenced by Rox1 binding affinity and Mot3 presence.

Conclusions:

  • Heme-mediated oxygen sensing is crucial for regulating gene expression in Saccharomyces cerevisiae.
  • The Rox1/Ssn6/Tup1 complex is a key repressor of hypoxic genes.
  • Mot3 enhances Rox1 repression, contributing to the differential regulation of hypoxic genes.

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