Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The metabolic hypothesis revisited.

C Rozanov1, A Roy, A Mokashi

  • 1Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia 19104-6085, USA.

Advances in Experimental Medicine and Biology
|June 13, 2000
PubMed
Summary

High carbon monoxide (CO) levels stimulate the chemosensory neurons (CSN) like hypoxia. Researchers pinpointed CO

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Critical behavior of a strongly interacting 2D electron system.

Physical review letters·2012
Same author

Paediatric adjustable strabismus surgery.

Eye (London, England)·2012
Same author

Bilateral disc swelling associated with posterior scleritis.

Eye (London, England)·2010
Same author

Improved membrane protein solubilization and clean-up for optimum two-dimensional electrophoresis utilizing GLUT-1 as a classic integral membrane protein.

Journal of neuroscience methods·2009
Same author

Preoperative site marking.

The British journal of ophthalmology·2009
Same author

Role of Fe2+ in oxygen sensing in the carotid body.

Advances in experimental medicine and biology·2004

Area of Science:

  • Biochemistry
  • Cellular Respiration
  • Mitochondrial Function

Background:

  • Hypoxia triggers a stimulatory response in chemosensory neurons (CSN).
  • High levels of carbon monoxide (CO) are used experimentally to mimic this hypoxia-induced CSN stimulation.
  • The precise mechanism of CO's stimulatory effect on CSN remains under investigation.

Purpose of the Study:

  • To elucidate the specific molecular target responsible for the stimulatory effects of high CO levels on CSN.
  • To investigate the role of mitochondrial respiration in mediating the response to CO.
  • To provide further evidence for the metabolic theory of oxygen sensing.

Main Methods:

  • Utilizing high concentrations of carbon monoxide (CO) to stimulate chemosensory neurons (CSN).

Related Experiment Videos

  • Employing light of varying wavelengths to probe the cellular response.
  • Analyzing the effects on the mitochondrial respiratory chain, specifically cytochrome c oxidase.

    • Main Results:

    • The stimulatory effects of high CO on CSN were found to be wavelength-dependent.
    • These wavelength-specific effects could be localized to cytochrome c oxidase within the mitochondrial respiratory chain.
    • This indicates that CO's action is directly linked to mitochondrial metabolic activity.

    Conclusions:

    • High CO mimics hypoxia-induced CSN stimulation by targeting cytochrome c oxidase.
    • The findings support the metabolic theory of oxygen sensing, emphasizing mitochondria's role.
    • Cytochrome c oxidase is a key component in sensing metabolic changes related to oxygen levels.