A macrophage receptor for apolipoprotein B48: cloning, expression, and atherosclerosis

¹Department of Medicine, Division of Gerontology and Geriatrics, University of Alabama, Birmingham, AL 35294-0012, USA.

Proceedings of the National Academy of Sciences of the United States of America

|June 15, 2000

Abstract:

We have cloned a human macrophage receptor that binds to apolipoprotein (apo)B48 of dietary triglyceride (TG)-rich lipoproteins. TG-rich lipoprotein uptake by the apoB48R rapidly converts macrophages and apoB48R-transfected Chinese hamster ovary cells in vitro into lipid-filled foam cells, as seen in atherosclerotic lesions. The apoB48R cDNA (3,744 bp) encodes a protein with no known homologs. Its approximately 3.8-kb mRNA is expressed primarily by reticuloendothelial cells: monocytes, macrophages, and endothelial cells. Immunohistochemistry shows the apoB48R is in human atherosclerotic lesion foam cells. Normally, the apoB48R may provide essential lipids to reticuloendothelial cells. If overwhelmed, foam cell formation, endothelial dysfunction, and atherothrombogenesis may ensue, a mechanism for cardiovascular disease risk of elevated TG.

Related Experiment Videos

A macrophage receptor for apolipoprotein B48: cloning, expression, and atherosclerosis

Related Concept Videos

Peroxisomal acetoacetyl-CoA thiolase of an n-alkane-utilizing yeast, Candida tropicalis.

Isolation and characterization of cDNAs from BamHI-H gene family RNAs associated with the tumorigenicity of Marek's disease virus.

Studies on antiplatelet agents. I. Synthesis and platelet inhibitory activity of 5-alkyl-2-aryl-4-pyridylimidazoles.

Physiological roles of acetoacetyl-CoA thiolase in n-alkane-utilizable yeast, Candida tropicalis: possible contribution to alkane degradation and sterol biosynthesis.

Analyses of the risk and operative stress for donors in living-related partial liver transplantation.

Cloning and characterization of the secY gene from the cyanobacterium Synechococcus PCC7942.

YBX1 regulates RNA polymerase III transcripts to prevent inflammation.

Advances in small area population estimation in the absence of national census data.

Co-option of ancestral stem regulators drove recurrent evolution of underground storage organs.

Profile of C. Robin Buell.

Urban tree canopy fragmentation and child mortality in low- and middle-income countries.

Loss of Sox10 prevents tumor initiation in vivo and induces luminal-to-basal reprogramming in Neu+ tumor cells.

Effect of bronchial thermoplasty on airway inflammatory and profibrotic gene expression after one-year follow-up in severe asthma.

Validation of a human peripheral neuronal model for ATP-P2X3 mediated inflammatory pain signalling in the dental pulp.

Novelty seeking and rapid symptom improvement across active and sham accelerated iTBS conditions: A pooled individual-patient data analysis.

Identification of the M1-like macrophage-associated diagnostic and therapeutic frameworks targeting carotid atherosclerosis patients: Integrative bioinformatic and multi-omic studies.

Intraoperative Guidewire Breakage During Proximal Femoral Nailing Managed Through Direct Anterior Approach: A Case Report and Review of Literature.

Matrix stress relaxation drives glioblastoma cell response in viscoelastic biomaterials.

Related Experiment Videos

A macrophage receptor for apolipoprotein B48: cloning, expression, and atherosclerosis

Abstract:

Related Concept Videos

Related Articles