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Related Experiment Videos

Genetic variability of hepatitis E virus within and between three epidemics in India.

R Aggarwal1, K A McCaustland, J B Dilawari

  • 1Hepatitis Branch, Division of Viral and Rickettsial Diseases, National Centerfor Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.

Virus Research
|June 15, 2000
PubMed
Summary

Genetic analysis of Hepatitis E virus (HEV) in India reveals significant variability between strains from different outbreaks and sporadic cases. Understanding this genetic diversity is crucial for tracking HEV epidemiology.

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Area of Science:

  • Virology
  • Molecular Epidemiology
  • Hepatology

Background:

  • Hepatitis E virus (HEV) is a major cause of acute viral hepatitis in developing nations.
  • India experiences both epidemic and sporadic HEV infections.

Purpose of the Study:

  • To evaluate the genetic variability of HEV strains from Indian outbreaks and sporadic cases.
  • To assess the utility of ORF1 and ORF2 gene segments for HEV molecular epidemiology.

Main Methods:

  • Sequencing of ORF1 (476 nt) and ORF2 (304 nt) regions of HEV specimens.
  • Analysis of genetic identity within and between outbreak strains.
  • Comparison with previously published Indian HEV sequences.

Main Results:

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  • High sequence identity (99.3-100.0%) observed within outbreaks.
  • Lower identity (97.1-99.2% for ORF1, 96.4-100.0% for ORF2) between outbreak strains.
  • Significant differences noted when comparing with prior Indian HEV sequences (95.2-98.5% for ORF1, 95.1-98.7% for ORF2).
  • One Meerut patient's strain and the Lucknow sporadic strain showed distinct clustering.

    • Conclusions:

    • ORF1 and ORF2 segments are valuable for studying HEV molecular epidemiology in India.
    • Considerable genetic variability exists among Indian HEV strains.
    • Further research is needed to fully understand the genetic diversity of HEV in India.