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Related Experiment Videos

Mouse receptor-activity-modifying proteins 1, -2 and -3: amino acid sequence, expression and function.

K Husmann1, P M Sexton, J A Fischer

  • 1Research Laboratory for Calcium Metabolism, Departments of Orthopaedic Surgery and Medicine, Zurich, Switzerland. khusmann@balgrist.unizh.ch

Molecular and Cellular Endocrinology
|June 16, 2000
PubMed
Summary

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Mouse receptor-activity-modifying proteins (RAMPs) 1, 2, and 3 were identified and characterized. Co-expression with calcitonin receptor-like receptor (CRLR) revealed distinct adrenomedullin (ADM) and calcitonin gene-related peptide (CGRP) receptors.

Area of Science:

  • Molecular biology
  • Receptor pharmacology
  • Genomics

Background:

  • Calcitonin receptor-like receptor (CRLR) requires receptor-activity-modifying proteins (RAMPs) for function.
  • RAMPs modulate CRLR to act as either an adrenomedullin (ADM) or a calcitonin (CT) gene-related peptide (CGRP) receptor.

Purpose of the Study:

  • To identify and characterize mouse RAMPs (mRAMPs).
  • To investigate the functional roles of mRAMPs in forming ADM and CGRP receptors with CRLR.

Main Methods:

  • Expressed sequence tags (ESTs) were used to identify mouse RAMP cDNA clones.
  • Northern blot analysis was performed to determine mRNA expression patterns of mRAMPs.
  • COS-7 cells were co-transfected with rat CRLR (rCRLR) and individual mRAMPs for binding and cAMP accumulation assays.

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Main Results:

  • Mouse RAMP1, -2, and -3 showed high amino acid sequence homology to human RAMPs.
  • mRAMP1, -2, and -3 exhibited distinct tissue expression patterns.
  • Co-expression studies demonstrated that mRAMP1 forms a CGRP receptor, mRAMP2 forms an ADM receptor, and mRAMP3 forms an ADM/CGRP receptor with rCRLR.

Conclusions:

  • Mouse RAMPs share significant homology with human counterparts.
  • Differential expression of mRAMPs suggests specific physiological roles.
  • The combination of CRLR with different mRAMPs generates distinct ADM and CGRP receptor subtypes.