Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Current concepts in neuroanatomical tracing.

C Köbbert1, R Apps, I Bechmann

  • 1Department of Experimental Ophthalmology, Medical School, University of Müenster, Germany.

Progress in Neurobiology
|June 17, 2000
PubMed
Summary

Neurobiology has been transformed by new tracing and cell labeling techniques. This review details essential neuroanatomical tracing methods for mapping central nervous system connections.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Changing Australia's trading language has enhanced the implementation of objective carcase measurement technologies.

Meat science·2024
Same author

Objective measurement technologies for transforming the Australian & New Zealand livestock industries.

Meat science·2021
Same author

Cannabinoid receptor CB2 ablation protects against TAU induced neurodegeneration.

Acta neuropathologica communications·2021
Same author

Why do anti-inflammatory signals of bone marrow-derived stromal cells improve neurodegenerative conditions where anti-inflammatory drugs fail?

Journal of neural transmission (Vienna, Austria : 1996)·2020
Same author

Globular glial tauopathy caused by MAPT P301T mutation: clinical and neuropathological findings.

Journal of neurology·2019
Same author

Muscarinic Receptor Modulation of the Cerebellar Interpositus Nucleus In Vitro.

Neurochemical research·2018

Area of Science:

  • Neurobiology
  • Neuroanatomy

Background:

  • Neurobiology has advanced significantly due to novel axonal tract tracing and cell labeling methods over the past 30 years.
  • Understanding the central nervous system's intricate connections is crucial for neurobiological research.

Purpose of the Study:

  • To review key neuroanatomical tracing methods currently employed in neurobiology.
  • To guide researchers in selecting appropriate techniques for their specific research interests.

Main Methods:

  • Overview of commonly used tracers: enzymes, peptides, biocytin, latex beads, plant lectins, and fluorescent dyes.
  • Detailed examination of established and novel neuroanatomical tracing techniques.
  • Discussion of technical aspects including tracer application, uptake, transport, and signal detection.

Related Experiment Videos

Main Results:

  • Specific methods discussed include in vivo fluorescent dye tracing, post-mortem tissue labeling, developmental studies, transcellular tracing, electrophysiological mapping with tract tracing, and multi-tracer detection.
  • Development of versatile three-color labeling protocols for complex connection studies.

Conclusions:

  • The review provides a comprehensive guide to neuroanatomical tracing techniques.
  • Effective selection and application of these methods are vital for advancing our understanding of neural circuitry.