Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A colorimetric detection system for Calymmatobacterium granulomatis.

J S Carter1, D J Kemp

  • 1Centre for Indigenous Natural and Cultural Resource Management, Faculty of Aboriginal, Northern Territory University, Darwin, Australia. j_carter@banks.ntu.edu.au

Sexually Transmitted Infections
|June 20, 2000
PubMed
Summary

A new colorimetric detection system using polymerase chain reaction (PCR) accurately identifies Calymmatobacterium granulomatis, enabling molecular diagnosis of donovanosis in routine labs. This assay offers a specific and sensitive method for detecting this granuloma-causing bacterium.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A phase I trial of paclitaxel, cisplatin, and veliparib in the treatment of persistent or recurrent carcinoma of the cervix: an NRG Oncology Study (NCT#01281852).

Annals of oncology : official journal of the European Society for Medical Oncology·2016
Same author

Incomplete offspring sex bias in Australian populations of the butterfly Eurema hecabe.

Heredity·2016
Same author

Analysis of Sarcoptes scabiei finds no evidence of infection with Wolbachia.

International journal for parasitology·2005
Same author

Scabies: more than just an irritation.

Postgraduate medical journal·2004
Same author

Genetic epidemiology of Sarcoptes scabiei (Acari: Sarcoptidae) in northern Australia.

International journal for parasitology·2004
Same author

Integrating pharmacokinetics knowledge into a drug ontology: as an extension to support pharmacogenomics.

AMIA ... Annual Symposium proceedings. AMIA Symposium·2004

Area of Science:

  • Molecular Biology
  • Diagnostic Microbiology

Background:

  • Donovanosis is a neglected tropical disease caused by Calymmatobacterium granulomatis.
  • Current diagnostic methods for donovanosis can be invasive and lack sensitivity.
  • There is a need for a rapid, specific, and accessible diagnostic assay for routine laboratory use.

Purpose of the Study:

  • To develop the first polymerase chain reaction (PCR) assay for Calymmatobacterium granulomatis.
  • To integrate this PCR assay into a colorimetric detection system.
  • To enable molecular diagnosis of donovanosis in routine diagnostic laboratories.

Main Methods:

  • A capture oligonucleotide specific for the Klebsiella phoE gene was immobilized on magnetic beads.
  • Biotinylated phoE PCR products from positive donovanosis specimens and various Klebsiella species were processed.

Related Experiment Videos

  • Differentiation and detection were achieved using enzymatic immunoassay (EIA) methodology after specific DNA cleavage and magnetic bead capture.

    • Main Results:

    • The developed colorimetric detection system demonstrated strong positive results for all 14 C. granulomatis positive specimens.
    • Isolates of Klebsiella pneumoniae, K. rhinoscleromatis, K. ozaenae, and samples from unrelated genital conditions tested negative.
    • The assay showed high specificity, distinguishing C. granulomatis from related bacteria.

    Conclusions:

    • A novel colorimetric detection system incorporating dual specificity for C. granulomatis has been successfully developed.
    • This system facilitates molecular diagnosis of donovanosis in routine laboratories, supporting public health initiatives.
    • Further validation with diverse global samples is recommended to confirm the generalizability of this diagnostic methodology.