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MS-2 fibrosarcoma characterization by laser induced autofluorescence
A Colasanti1, A Kisslinger, G Fabbrocini
1Istituto Nazionale di Fisica della Materia--Unità di Napoli, Italy. colasant@cds.unia.it
Lasers in Surgery and Medicine
|June 22, 2000
Summary
Autofluorescence (AF) analysis of MS-2 fibrosarcoma in mice revealed lower intensity and faster decay in tumor tissue compared to healthy tissue. This suggests the presence of free NADH in the tumor.
Area of Science:
- Biophysics
- Cancer Research
- Biophotonics
Background:
- Autofluorescence (AF) spectroscopy is a non-invasive technique for tissue analysis.
- MS-2 fibrosarcoma in BALB-CDF1 mice serves as a model for studying tumor characteristics.
Purpose of the Study:
- To investigate the autofluorescence properties of MS-2 fibrosarcoma using frequency and time domain measurements.
- To differentiate between healthy and tumoral tissue based on autofluorescence emission spectra and decay kinetics.
Main Methods:
- Excitation of autofluorescence using a nitrogen (N(2)) laser beam (337.1 nm).
- Acquisition of steady-state AF spectra using a spectrograph and multichannel detection.
- Measurement of time-resolved fluorescence decay using a monochromator and photomultiplier system.
Main Results:
- Pathologic (tumoral) tissue exhibited lower autofluorescence intensity than healthy tissue in the 400-500 nm spectral region.
- Fluorescence decay in the 400-500 nm region consisted of both fast and slow components.
- The fast fluorescence decay component's lifetime was significantly shorter in tumoral tissue compared to healthy samples.
Conclusions:
- Combined frequency and time domain AF measurements effectively characterize MS-2 fibrosarcoma.
- The observed autofluorescence characteristics suggest the probable presence of the free form of NADH in MS-2 fibrosarcoma.