Testosterone in human urine as determined by gas-liquid chromatography

A simple and accurate gas chromatographic method for the determination of testosterone in human urine is described. Radioactive testosterone was added to samples to correct methodological loss. A mild acid hydrolysis under protection of benzene layer gave a single estimate of testosterone glucuronate and sulphate. Paper chromatography and rechromatography on chromatoplate served for preliminary qurification. Testosterone was gas chromatographed as acetate and guantitated by the internal standard procedure and flame ionization detector. Results of control experiments, normal values for female and male subjects and estimations in patients with alterations of androgen hormone metabolism are presented.