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[Predominance of phage group II in Staphylococcus strains isolated from humans]
E Krzywińska1, L Piechowicz, J Galiński
1Zakład Mikrobiologii Lekarskiej Akademii Medycznej w Gdańsku.
Abstract:
2545 strains of Staphylococcus aureus isolated from 24 Polish laboratories in the years 1994-95 were investigated. Phage typing was performed according to the method of Blair and Williams using the present basic set of typing phages and additional phages 88, 89 and 187. The phages were employed in concentrations of RTD and 100 x RTD. The predominance of phage group II, reported elsewhere since 1980-ties, was found in the present study (23.6%). Strains of group III were second in frequency (16.6%), whereas strains of groups I and V, as well as type 95 occurred in small percentage (7.6%, 4% and 3.4% respectively). Strains of groups II and V have been rarely lysed by phages belonging to other groups. The use of additional phages resulted in typability of strains by 7.9%. Percentage of non-typable strains was high and amounted to 22.0%.
Insights
This study analyzed 2545 Staphylococcus aureus strains from Poland. Phage typing revealed a predominance of phage group II (23.6%), highlighting key bacterial typing trends.
Area of Science:
- Microbiology
- Bacteriology
- Epidemiology
Context:
- Investigated 2545 Staphylococcus aureus strains collected from 24 Polish laboratories between 1994-1995.
- Phage typing was conducted using the Blair and Williams method with standard and additional phages (88, 89, 187) at RTD and 100 x RTD concentrations.
Purpose:
- To determine the phage typing patterns of Staphylococcus aureus strains prevalent in Poland during the mid-1990s.
- To assess the effectiveness of the standard phage set and additional phages in typing these strains.
Summary:
- Phage group II was predominant (23.6%), followed by group III (16.6%). Strains from groups I, V, and type 95 were less frequent.
- A significant percentage of strains (22.0%) remained non-typable, indicating challenges in current phage typing methodologies.
- The addition of specific phages improved typability by 7.9%.
Impact:
- Provides epidemiological data on Staphylococcus aureus phage types in Poland during the specified period.
- Highlights the need for updated or expanded phage typing sets to improve the characterization of Staphylococcus aureus strains.
- Contributes to understanding antimicrobial resistance patterns and infection control strategies.