Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Actin depolymerization and polymerization are required during apoptosis in endothelial cells.

N Suarez-Huerta1, R Mosselmans, J E Dumont

  • 1Institut de Recherche Interdisciplinaire en Biologie Humaine et Nucléaire (IRIBHN), Faculté de Médecine, Free University of Brussels (U.L.B.), Brussels, Belgium. nsuarez@ulb.ac.be

Journal of Cellular Physiology
|June 27, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development and validation of an in vitro model to study thrombin generation on the surface of catheters in platelet-poor and platelet-rich plasma.

Thrombosis research·2024
Same author

3D reconstruction of the left atrium for precise localisation and ablation of accessory pathway.

Acta cardiologica·2022
Same author

Contrast ultrasound for the quantification of deep vein thrombosis in living mice: effects of enoxaparin and P2Y12 receptor inhibition.

Journal of thrombosis and haemostasis : JTH·2013
Same author

Extracellular nucleotide derivatives protect cardiomyocytes against hypoxic stress.

Biochemical pharmacology·2011
Same author

The purinergic receptor P2Y2 receptor mediates chemotaxis of dendritic cells and eosinophils in allergic lung inflammation.

Allergy·2010
Same author

Distal colonic Na(+) absorption inhibited by luminal P2Y(2) receptors.

Pflugers Archiv : European journal of physiology·2007
Same journal

Mt1-Ca<sup>2+</sup>-Mitochondrial Metabolic Axis Maintains Temporomandibular Joint Condylar Cartilage Homeostasis Under Low Oxygen and Hypoxic Condition.

Journal of cellular physiology·2026
Same journal

Correction to "IRE1α/NOX4 Signaling Pathway Mediates Ros-Dependent Activation of Hepatic Stellate Cells in NaAsO<sub>2</sub>-Induced Liver Fibrosis".

Journal of cellular physiology·2026
Same journal

Lipocalin-2 Restores Mitochondrial and Antioxidant Adaptation in a Strain-Specific Manner During LPS Induced Sepsis.

Journal of cellular physiology·2026
Same journal

Dysregulation of Non-Muscle Myosin IIA Assembly and Phosphorylation in S100A4 Null Mouse Lens.

Journal of cellular physiology·2026
Same journal

Corrigendum to "A Probiotic Combination of Limosilactobacillus reuteri and Clostridium butyricum Ameliorates 5-Fluorouracil-Induced Intestinal Mucositis in Rats by Strengthening Barrier Integrity and Modulating Immuno-Microbial Homeostasis".

Journal of cellular physiology·2026
Same journal

Sexually Dimorphic Regulation of MiR-29a/c-3p in Human Endothelial Cells: Cell Functions and Transcriptome.

Journal of cellular physiology·2026
See all related articles

Actin microfilaments disrupt during apoptosis, but actin re-organization is essential for bleb formation and cell fragmentation. This study reveals a dual role for actin in programmed cell death.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Actin microfilaments are crucial cytoskeletal components involved in various cellular processes.
  • Apoptosis, or programmed cell death, involves significant cellular morphological changes.

Purpose of the Study:

  • To elucidate the role of actin microfilaments in tumor necrosis factor-alpha (TNF)-induced apoptosis in bovine aortic endothelial (BAE) cells.
  • To investigate the dynamic changes in actin organization during the apoptotic process.

Main Methods:

  • Western blotting analysis to assess protein levels.
  • Immunofluorescence microscopy to visualize actin microfilament networks.
  • Treatment with cytochalasin to disrupt actin polymerization.

Related Experiment Videos

Main Results:

  • Actin microfilament network disruption was observed in apoptotic BAE cells.
  • F-actin depolymerization correlated with DNA degradation and morphological changes.
  • Actin polymerization, sensitive to cytochalasin, was required for bleb formation and apoptotic fragmentation.
  • Cytochalasin E reversed incipient apoptotic fragmentation and actin accumulated at apoptotic bodies.

Conclusions:

  • Actin network disruption occurs concurrently with apoptotic morphological changes.
  • Cytochalasin-sensitive actin reorganization is essential for the formation of apoptotic blebs and cell fragmentation.