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Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor (LATS) Biosensor
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Hippo Pathway Regulation and Microenvironmental Cues Governing Human MSC Transition to Cancer-Associated Fibroblasts

Pimjai Chingsuwanrote1, Chanchao Lorthongpanich1,2, Pakpoom Kheolamai3

  • 1Department of Medicine, Siriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.

Journal of Cellular Physiology
|August 5, 2026
PubMed
Summary

Three-dimensional culture, hypoxia, and cancer factors transform human mesenchymal stem cells (hMSCs) into inflammatory cancer-associated fibroblasts (iCAFs) by down-regulating YAP/TAZ. This YAP/TAZ regulation is key for hMSC-to-CAF transformation in tumor stroma.

Keywords:
3‐dimensional (3D) cultureYAPcancer associated fibroblastshypoxic conditionmesenchymal stem cells

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