Studies of mouse-brain-associated T-lymphocyte antigens

Xeno- and alloantigens shared by mouse brain, thymocytes and thymus-derived lymphocytes were isolated from brain by butanol extraction, followed by Sephadex G-200 column chromatography and preparative polyacrylamide gel electrophoresis, both in the presence of SDS. The antigenic activity, as tested by inhibition of complement-mediated cytotoxicity using a xenogeneic antiserum directed against T cell-specific antigens (anti-MTLA), was enriched 50-fold during the purification procedure. Xenogeneic and allogeneic antisera which were specific for mouse T-lymphocytes, could be obtained by immunization of rabbits and mice with the partially purified antigens.