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Secondary structure of the 5'-region of PGY1/MDR1 mRNA
E V Kostenko1, R S Beabealashvilly, V V Vlassov
1Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of Rusian Academy of Sciences, Novosibirsk 630090, Russia.
Abstract:
In order to identify the optimal target sites for antisense oligonucleotides in the human multiple drug resistance mRNA, the secondary structure of the 5'-terminal part of this mRNA (nucleotides 1-678) was investigated. By using results of probing with ribonucleases T1, ONE and V1 and results of computer simulations, a model of the 5'-region of the PGY1/MDR1 mRNA was built. The molecule is formed by three major domains comprising several hairpins separated by single-stranded fragments. The predicted single-stranded regions of the PGY1/MDR1 mRNA efficiently bind complementary oligonucleotides.