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Related Experiment Videos

The anaphase-promoting complex: new subunits and regulators.

A M Page1, P Hieter

  • 1Center for Molecular Medicine and Therapeutics, University of British Columbia, Vancouver, Canada. apage@cmmt.ubc.ca

Annual Review of Biochemistry
|June 29, 2000
PubMed
Summary

The anaphase-promoting complex (APC) controls cell division by degrading regulatory proteins. New research reveals intricate mechanisms governing APC regulation, including specificity factors and self-degradation, suggesting conserved ubiquitination pathways.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Ubiquitin-mediated proteolysis is essential for cell cycle regulation.
  • The anaphase-promoting complex (APC) is a key E3-ubiquitin ligase controlling cell division by targeting proteins for destruction.
  • Understanding APC regulation is critical for comprehending cell cycle control.

Purpose of the Study:

  • To elucidate the intricate mechanisms governing the regulation of the anaphase-promoting complex (APC).
  • To explore how specificity factors and APC-mediated proteolysis contribute to temporal control of substrate degradation.
  • To investigate potential similarities in substrate ubiquitination mechanisms between the APC and SCF E3 ubiquitin-ligase complex.

Main Methods:

  • Review and synthesis of recent findings on APC subunits and regulatory proteins.

Related Experiment Videos

  • Analysis of conserved sequence motifs (WD-40 repeats, cullin homology domains) in APC and SCF complexes.
  • Comparative examination of regulatory mechanisms in ubiquitin-mediated proteolysis.

    • Main Results:

    • Identification of specificity factors as a key mechanism for temporal control of substrate degradation by the APC.
    • Demonstration that the APC can mediate the proteolysis of its own regulators.
    • Observation of conserved sequence motifs in both APC and SCF complexes, suggesting shared ubiquitination strategies.

    Conclusions:

    • APC regulation involves sophisticated mechanisms including specificity factors and autoregulation.
    • Conserved structural motifs suggest convergent or divergent evolution of ubiquitination pathways in related E3 ligase complexes.
    • Further research into these mechanisms will enhance our understanding of cell cycle control and protein degradation.