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Related Experiment Videos

Flow cytometric analysis of microorganisms.

M K Winson1, H M Davey

  • 1Institute of Biological Sciences, University of Wales, Aberystwyth, SY23 3DD, United Kingdom.

Methods (San Diego, Calif.)
|June 30, 2000
PubMed
Summary
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Flow cytometry, once underused in microbiology, is now revolutionizing the field. Advances in bioinformatics and reporter genes enable new applications in microbial gene regulation and directed evolution.

Area of Science:

  • Microbiology
  • Biotechnology
  • Molecular Biology

Background:

  • Flow cytometry, a powerful cell analysis technique, has been historically underutilized in microbial research.
  • Recent technological advancements in instrumentation, bioinformatics, and reporter gene systems are driving renewed interest and application in microbiology.

Purpose of the Study:

  • To review the historical underdevelopment of flow cytometry in microbiology.
  • To highlight novel and emerging applications of flow cytometry in microbial investigations.
  • To discuss the limitations and future strategies for flow cytometry in microbiology.

Main Methods:

  • Review of existing literature on flow cytometry applications in microbiology.
  • Analysis of recent technological advancements, including reporter gene technologies and bioinformatics.

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  • Examination of specific applications such as viability studies, taxonomy, gene regulation, and directed evolution.

    • Main Results:

    • Flow cytometry is increasingly recognized for its utility in microbial studies.
    • Novel applications include studying host-pathogen interactions using green fluorescent protein variants and isolating mutants via fluorescence-activated cell sorting for directed evolution.
    • Significant progress has been made in overcoming previous limitations.

    Conclusions:

    • Flow cytometry offers significant potential for advancing microbiological research.
    • Continued development in technology and methodology will further expand its applications.
    • Microbiologists are well-positioned to leverage flow cytometry for novel discoveries.