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pUB2380: characterization of a ColD-like resistance plasmid
B Albiger1, A Comanducci, H M Dodd
1Laboratoire de Microbiologie et de Génétique, UPRES A 7010-CNRS, 28, rue Goethe, Strasbourg Cedex, 67083, France.
Plasmid
|June 30, 2000
Summary
This study details the ColE1-like resistance plasmid pUB2380, revealing its hybrid structure. Analysis shows it encodes replication, mobilization, and kanamycin resistance functions, likely originating from enterobacteriaceae.
Area of Science:
- Microbiology
- Molecular Biology
- Genetics
Background:
- ColE1-like plasmids are crucial in bacterial genetics and antibiotic resistance.
- Understanding plasmid structure and function aids in combating antimicrobial resistance.
Purpose of the Study:
- To conduct a comprehensive analysis of the mobilizable, ColE1-like resistance plasmid pUB2380.
- To elucidate the genetic organization and potential origins of pUB2380.
Main Methods:
- Genomic analysis of the 8.5-kb plasmid pUB2380.
- Identification and characterization of encoded functions and genetic elements.
- GC content analysis to infer origins of different genomic regions.
Main Results:
- The plasmid pUB2380 genome encodes six to seven major functions.
- Key functions include replication (oriV, RNAI, RNAII), mobilization (oriT), multimer resolution (cer), and kanamycin resistance (aph).
- GC content suggests a hybrid structure derived from at least three sources, with replication, mobilization, and resistance genes likely from enterobacteriaceae.
Conclusions:
- Plasmid pUB2380 possesses a complex, hybrid genomic structure.
- Its functional components are homologous to known elements in E. coli and related bacteria.
- The findings contribute to understanding plasmid evolution and dissemination of antibiotic resistance.