Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A method for demonstrating gene essentiality in Staphylococcus aureus.

M Jana1, T T Luong, H Komatsuzawa

  • 1Department of Microbiology, University of Kansas Medical Center, Kansas City, Kansas 61660, USA.

Plasmid
|June 30, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of hyperthermia on intracellular pH in human U-87 MG glioblastoma cells.

Oncology·1995
Same author

Purified rat acid-labile subunit and recombinant human insulin-like growth factor (IGF)-binding protein-3 can form a 150-kilodalton binary complex in vitro in the absence of IGFs.

Endocrinology·1995
Same author

Molecular analysis of an extracellular protease gene from Vibrio parahaemolyticus.

Microbiology (Reading, England)·1995
Same author

Set-shifting aptitude in Parkinson's disease: external versus internal cues.

Psychological reports·1995
Same author

Luminal epidermal growth factor is trophic to the small intestine of parenterally fed rats.

Clinical science (London, England : 1979)·1995
Same author

Comparative mathematical analyses of freezing in lung and solid tissue.

Cryobiology·1995

The murE gene is essential for Staphylococcus aureus growth. This study developed a method to demonstrate gene essentiality, confirming murE

Area of Science:

  • Microbiology
  • Molecular Biology
  • Bacterial Genetics

Background:

  • Peptidoglycan synthesis is crucial for bacterial cell wall integrity.
  • Identifying essential genes in Staphylococcus aureus is vital for understanding bacterial physiology and developing new antimicrobials.

Purpose of the Study:

  • To establish a method for determining gene essentiality in Staphylococcus aureus.
  • To ascertain if the murE gene, involved in peptidoglycan synthesis, is essential for S. aureus growth.

Main Methods:

  • Constructed a S. aureus strain (CYL368) with the murE gene under inducible spac promoter control.
  • Utilized a plasmid (pMJ8426) expressing the E. coli lacI gene to regulate murE expression.
  • Assessed bacterial growth in the presence and absence of the inducer isopropyl-beta-d-thiogalactopyranoside.

Related Experiment Videos

Main Results:

  • The engineered S. aureus strain (CYL368(pMJ8426)) exhibited normal growth with the inducer.
  • The strain failed to grow without the inducer, indicating dependence on the regulated murE gene.
  • Demonstrated that the murE gene is indispensable for S. aureus growth in rich medium.

Conclusions:

  • The developed method effectively demonstrates gene essentiality in S. aureus.
  • The murE gene is confirmed as an essential gene for Staphylococcus aureus viability.
  • This finding contributes to the knowledge of essential bacterial pathways and potential therapeutic targets.