Related Experiment Video
Updated: Aug 8, 2026
A Method to Study the Impact of Chemically-induced Ovarian Failure on Exercise Capacity and Cardiac Adaptation in Mice
Published on: April 7, 2014
Nicotine induced ovarian and uterine changes in albino mice
Abstract:
Nicotine at the dose level of 0.3 mg/100 g body weight was administered to normal cycling mice for 15 days through oral and intraperitoneal routes. At autopsy on 16th day significant reduction in the ovarian and uterine weight was observed. Histological observations showed decrease in the number and size of Graafian follicles, corpora lutea and increase in the atretic follicles in the ovary. The uterus showed absence of endometrial glands, decrease in the height of myometrium, endometrium and its epithelial cells. The total cholesterol content of the ovary and uterus is increased whereas the protein content is decreased. This antagonistic action of nicotine to gonadotrophins is discussed.
Insights
Nicotine exposure in mice significantly reduced ovarian and uterine weight, impacting reproductive health. This study highlights nicotine
Area of Science:
- Reproductive Toxicology
- Endocrinology
Background:
- Nicotine is a primary psychoactive compound in tobacco products.
- Its effects on reproductive organs require further elucidation.
Purpose of the Study:
- To investigate the impact of chronic nicotine administration on the reproductive system of female mice.
- To analyze the histological and biochemical changes in the ovary and uterus.
Main Methods:
- Female mice were administered nicotine (0.3 mg/100 g body weight) daily for 15 days via oral and intraperitoneal routes.
- Ovarian and uterine weights were measured, and histological examinations were performed.
- Biochemical analysis of cholesterol and protein content in reproductive organs was conducted.
Main Results:
- Significant reductions in ovarian and uterine weights were observed.
- Histological analysis revealed decreased Graafian follicles and corpora lutea, with increased atretic follicles in ovaries.
- Uterine tissues showed atrophied endometrial glands, reduced myometrial/endometrial height, and decreased epithelial cell size.
- Ovarian and uterine cholesterol levels increased, while protein content decreased.
Conclusions:
- Nicotine exposure antagonizes gonadotropin function, leading to adverse effects on female reproductive organs.
- These findings suggest nicotine disrupts normal ovarian and uterine physiology, potentially impacting fertility.
