[Haplotype identification of single sperm in pigs]

Haplotype of porcine single sperm was analyzed by PCR method through using primer extension preamplification and heminesting primer design strategy. The PCR products were run on 8% polyacrylamide gel, and visualized by silver staining method. The results showed that the haplotypes of individual sperm can be unequivocally demonstrated by these methods. The application of single sperm typing provides a unique tool for the construction of high resolution genetic map and the study of genetic phenomena requiring large sample size.