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Updated: Aug 10, 2026
Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing
Published on: March 31, 2017
[Identification of molecular markers linked to the Pm6 gene conferring powdery mildew resistance in wheat] [In
1Key Open Lab of Agri. Ministry for Crop Cytogenetics, Nanjing Agricultural University, Jiangsu.
Abstract:
The Pm6 gene from T. timopheevi confers resistance to wheat Erysiphe graminis f. sp. tritici. A total of 8 RFLP markers were selected for their ability to reveal polymorphism on powdery mildew resistant 'Prins' near-isogenic lines (with Pm6) and the recurrent parent Prins. The linkage relationship between the polymorphic markers and Pm6 gene was estimated with a F2 population from cross of NIL with Prins. It was indicated that three markers Xbcd135EcoRV9kb, Xbcd307EcoR18.8kb and Xbcd266EcoRV18kb are closely linked to the gene locus with distances 1.6 +/- 1.5cM, 1.6 +/- 1.5cM, 4.8 +/- 2.6cM, respectively. Eigteen wheat cultivars or lines reported as containing Pm6 genes were employed to assess the applicability of the three markers for identification of Pm6 gene in different genetic background. Results showed that marker Xbcd135-2BL can be used to tag the presence of Pm6 gene introgressed to different wheat genetic background.
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