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Updated: Aug 5, 2026

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE
Published on: October 16, 2017
Binding of barley and wheat alpha-thionins to polysaccharides
S Oita1, M Ohnishi-Kameyama, T Nagata
1Shikoku National Agricultural Experiment Station, Ministry of Agriculture, Forestry and Fisheries, Zentsuji, Kagawa, Japan. oita@skk.affrc.go.jp
Abstract:
An antimicrobial peptide termed BCP-2 was purified from barley grain by chitin-affinity treatment and HPLC. The results of amino acid analysis and mass spectrometry of BCP-2 indicate that the peptide is very similar to barley alpha-thionin. BCP-2 and wheat alpha1-thionin were also bound to beta-glucan but not to starch. The binding of BCP-2 to laminarin (beta-1,3-1,6-glucan) and laminarioligosaccharides was supported by fluorescence polarization data. This is the first report on the binding of alpha-thionins to polysaccharide containing chitin and beta-1,3-glucan, which construct fungal cell walls.
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