[Functional and structural analysis of a prokaryotic enhancer-like element in Escherichia coli MC1061 strain]

Cat and lacZ genes were used as reporter gene and three prokaryotic enhancer-like element (MC2, MC8 and MC9) were identified in the genomic DNA of MC1061 strain. All three fragments can improve the expression of lacZ gene by 2-5 times with the orientation independence. The results of in vivo transcription and Dot blot hybridization assays suggested that MC8 regulated the expression of lacZ at transcription level. Stepwise deletion expreriments showed the functional domain of MC8 located at 450-950 bp, and in regions 450-600 bp and 840-950 bp contain at least one functional loci. Sequence data indicated three are 3 A + T rich sections in MC8, 2 of them are in the functional loci.