[Cloning of human TRAIL cDNA and its expression in COS-7 cells]

The human TRAIL cDNA was amplified with the total RNA from the human acute promyelocytic leukemia cell line HL-60 by means of RT-PCR, and was cloned into the pGEM-T vector. The DNA sequence analysis showed that it was consistent with the published sequence. Then, the insert of human TRAIL cDNA was subcloned into the mammalian expression vector pcDNA3. The hybrid plasmid pcDNA3-hTRAIL was transformed into COS-7 cells, and transiently expressed in the COS-7 cells. The activity of the expressed product could induce apoptosis in U937 cell line.