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Single polyprenol and dolichol isolation by semipreparative high-performance liquid chromatography technique
T Carlson1, K Skorupinska-Tudek, J Hertel
1Department of Immunology, Microbiology, Pathology, and Infectious Diseases, Karolinska Institute, Division of Pathology, Huddinge University Hospital, Sweden.
Abstract:
A new method of separation of single polyprenols (or dolichols) from a mixture of isoprenoid alcohols is described. Application of a high-performance liquid chromatography (HPLC) apparatus equipped with a semipreparative ODS column resulted in preparation of long-chain (dihydro)polyprenols of high purity (>95%). This approach substantially decreases the time scale of the conventional chromatographical preparative procedure. The method can be widely used in chemical and biochemical projects, where single polyprenols or dolichols are required.