Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sequence-based analysis of mutagenized mice.

D R Beier1

  • 1Genetics Division, Brigham and Women's Hospital, 20 Shattuck Street, Boston, Massachusetts 02115, USA. beier@rascal.med.harvard.edu

Mammalian Genome : Official Journal of the International Mammalian Genome Society
|July 25, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A mutation in Tubb2b, a human polymicrogyria gene, leads to lethality and abnormal cortical development in the mouse.

Human molecular genetics·2013
Same author

Focusing forward genetics: a tripartite ENU screen for neurodevelopmental mutations in the mouse.

Genetics·2011
Same author

Identification of a Van der Woude syndrome mutation in the cleft palate 1 mutant mouse.

Genesis (New York, N.Y. : 2000)·2010
Same author

Ttc21b is required to restrict sonic hedgehog activity in the developing mouse forebrain.

Developmental biology·2009
Same author

Genome-wide SNP analysis of Tg.AC transgenic mice reveals an oncogenic collaboration between v-Ha-ras and Ink4a, which is absent in p53 deficiency.

Oncogene·2007
Same author

Impaired homeostasis and phenotypic abnormalities in Prdx6-/-mice lens epithelial cells by reactive oxygen species: increased expression and activation of TGFbeta.

Cell death and differentiation·2005
Same journal

Correction: Are we fully exploiting genetic discoveries to understand and treat Alzheimer's disease?

Mammalian genome : official journal of the International Mammalian Genome Society·2026
Same journal

Neuroinflammation in glaucoma: a myriad of cellular pathways and players.

Mammalian genome : official journal of the International Mammalian Genome Society·2026
Same journal

Correction: THBS1: a biomarker for PCOS and its role in pathogenesis via the PI3K/AKT signaling pathway.

Mammalian genome : official journal of the International Mammalian Genome Society·2026
Same journal

Elucidating the therapeutic targets and multi-target mechanisms of salvianolic acid A for diabetic nephropathy.

Mammalian genome : official journal of the International Mammalian Genome Society·2026
Same journal

DeepDisSNP: Predicting disease-associated SNPs by representation learning on disease and SNP linkage disequilibrium networks.

Mammalian genome : official journal of the International Mammalian Genome Society·2026
Same journal

A comprehensive full-length transcriptome atlas across multiple organs of an Indian yak breed-Arunachali.

Mammalian genome : official journal of the International Mammalian Genome Society·2026
See all related articles

Ethylnitrosourea (ENU) effectively mutagenizes mouse spermatogonial cells. PCR-based mutation detection offers a faster, more efficient alternative to traditional methods for assessing ENU treatment efficacy.

Area of Science:

  • Genetics
  • Molecular Biology
  • Toxicology

Background:

  • Ethylnitrosourea (ENU) is a potent mutagen used to induce mutations in mouse spermatogonial cells for genetic screens.
  • Traditional methods for assessing mutagenesis efficiency, like the specific locus test, are costly, time-consuming, and logistically challenging.

Purpose of the Study:

  • To develop and validate a more efficient method for assessing the efficiency of ethylnitrosourea (ENU) mutagenesis in mice.
  • To establish a rapid assessment of mutagen treatment efficacy using post-treatment progeny analysis.

Main Methods:

  • Utilizing Polymerase Chain Reaction (PCR)-based mutation detection techniques.
  • Analyzing progeny from ENU-treated mice after a single generation of breeding.

Related Experiment Videos

Main Results:

  • PCR-based mutation detection provides a rapid assessment of mutagen treatment efficiency.
  • Theoretical and empirical analyses suggest a per-base mutation frequency of approximately 1 sequence change per 10^5 base pairs for fractionated-dose ENU treatment.

Conclusions:

  • PCR-based mutation detection is a valuable tool for efficiently assessing ENU mutagenesis in mice.
  • This strategy can be broadly applied for systematic determination of gene function through mutagenesis screens.