Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

B-cell function in canine X-linked severe combined immunodeficiency.

B J Hartnett1, R L Somberg, S Krakowka

  • 1Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, 3850 Spruce St., Philadelphia, PA, USA.

Veterinary Immunology and Immunopathology
|July 13, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characterization of a novel functional porcine CD3<sup>+</sup>CD4<sup>low</sup>CD8α<sup>+</sup>CD8β<sup>+</sup> T-helper/memory lymphocyte subset in the respiratory tract lymphoid tissues of swine influenza A virus vaccinated pigs.

Veterinary immunology and immunopathology·2024
Same author

Correction: A split influenza vaccine formulated with a combination adjuvant composed of alpha-D-glucan nanoparticles and a STING agonist elicits cross-protective immunity in pigs.

Journal of nanobiotechnology·2022
Same author

A split influenza vaccine formulated with a combination adjuvant composed of alpha-D-glucan nanoparticles and a STING agonist elicits cross-protective immunity in pigs.

Journal of nanobiotechnology·2022
Same author

Evaluation of the Potency, Neutralizing Antibody Response, and Stability of a Recombinant Fusion Protein Vaccine for Streptococcus pyogenes.

The AAPS journal·2017
Same author

7th International Immunoglobulin Conference: Immunodeficiencies.

Clinical and experimental immunology·2014
Same author

Common variable immunodeficiency (CVID): new genetic insight and unanswered questions.

Clinical and experimental immunology·2014
Same journal

Early in vitro response to Toxoplasma gondii infection in macrophages and neutrophils from sheep immunized with a commercial vaccine.

Veterinary immunology and immunopathology·2026
Same journal

Host immune response to lumpy skin disease virus.

Veterinary immunology and immunopathology·2026
Same journal

A randomized field trial of live epizootic bovine abortion agent (EBAA) vaccine demonstrates safety and efficacy.

Veterinary immunology and immunopathology·2026
Same journal

Comparative evaluation of lateral flow immunoassay and real time PCR for detection of canine morbillivirus antigen in live attenuated vaccines.

Veterinary immunology and immunopathology·2026
Same journal

Establishment of a disease model for Mycoplasma Ovipneumoniae infectioninfection in Hu sheep and its lung transcriptomic analysis.

Veterinary immunology and immunopathology·2026
Same journal

Macrophage-mediated inflammatory responses impair mucosal barrier components and promote goblet cell loss during Eimeria tenella infection in chickens.

Veterinary immunology and immunopathology·2026
See all related articles

Canine X-linked severe combined immunodeficiency (XSCID) involves mutations in the common gamma (gammac) subunit. XSCID dogs exhibit distinct B-cell responses compared to mice, offering a valuable model for cytokine research.

Area of Science:

  • Immunology
  • Genetics
  • Comparative Medicine

Background:

  • Canine X-linked severe combined immunodeficiency (XSCID) shares clinical similarities with human XSCID.
  • Mutations in the common gamma (gammac) subunit affect cytokine receptor signaling.
  • Previous studies highlighted profound T-cell development defects in XSCID dogs compared to gamma c-deficient mice.

Purpose of the Study:

  • To evaluate B-cell function in dogs with X-linked severe combined immunodeficiency.
  • To compare canine XSCID B-cell characteristics with those of XSCID humans and gamma c-deficient mice.
  • To assess the utility of the XSCID dog as a model for studying gamma c-dependent cytokine roles in B-cell development.

Main Methods:

  • Analysis of peripheral B-cell proportions and numbers in XSCID dogs.

Related Experiment Videos

  • Assessment of B-cell proliferation in response to T-cell-dependent (pokeweed mitogen) and T-cell-independent (Staphylococcus aureus) mitogens.
  • Evaluation of immunoglobulin class-switching capabilities (IgM to IgG) in vitro and in vivo.

    • Main Results:

    • XSCID dogs show increased peripheral B-cell numbers, unlike gamma c-deficient mice.
    • Canine XSCID B-cells proliferate to T-cell-independent stimuli but not T-cell-dependent stimuli.
    • XSCID dogs produce IgM but exhibit impaired class-switching to IgG production, mirroring XSCID boys.

    Conclusions:

    • Gamma c-dependent cytokines play differential roles in B-cell development across species (dogs, humans vs. mice).
    • The canine XSCID model provides unique insights into B-cell development and function.
    • This model is valuable for investigating the impact of gamma c-dependent cytokines on B-cell immunity.