Observations regarding the neurocytokaryological study of the experimental infection with herpes simplex virus type 1...

Mice distributed in groups were inoculated with the herpes virus simplex type 1 and type 2 strains in various dilutions (10(-1)-10(-7)), which had been maintained on primary human embryo cell cultures. The animals were killed and homogenates were prepared of their brains, which were used for testing the infective titre in vivo and for the isolation of nuclei, by means of the Hymer-Kuff technique. For the cytokaryological examinations, smears were prepared from the homogenate, which were stained by May-Grünwald-Giemsa and Mann methods. The cell and nucleus types were evaluated morphologically, descriptively, according to the size and structure. On the basis of three criteria the following groups were set up: large sized cells with bulky nuclei; middle sized cells with nuclei of a medium size; small sized cells with small nuclei and visible nucleoli; small sized cells with small, tachychromatic nuclei; cells exhibiting karyolysis. In both the infection with herpes virus simplex type 1 and type 2, the small sized cells with small tachychromatic nuclei represent the highest rate. Between the variants of the elaborated models no significant differences regarding the cell and nucleus types are recorded. On the whole, the cytokaryological picture does not exhibit notable differences, but rather resemblances with respect to the proportionality of the nucleus types in the experimental variants studied, in the case of herpes simplex virus type 1 and 2 induced infections.