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One step purification of peanut phospholipase D by precipitation with alginate
S Sharma1, A Sharma, M N Gupta
1Chemistry Department, Indian Institute of Technology, New Delhi, India.
Bioseparation
|July 13, 2000
Abstract:
A simple titrimetric assay with soybean lecithin has been used for screening phospholipase D activity from some plant sources, viz. peanut, wheat germ, cabbage and carrot. The enzyme from peanut has been purified by binding to alginate which is a water soluble polymer. The purification consisted of co-precipitation of enzyme with alginate upon addition of 0.06 M Ca++. The enzyme was eluted from the polymer using 0.2 M sodium chloride. The activity recovery was 61% with 34 fold purification.