Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Integrin alpha(3)-subunit expression modulates alveolar epithelial cell monolayer formation.

R L Lubman1, X L Zhang, J Zheng

  • 1Division of Pulmonary and Critical Care Medicine and Will Rogers Institute Pulmonary Research Center, University of Southern California, Los Angeles, California 90033, USA. rlubman@hsc.usc.edu

American Journal of Physiology. Lung Cellular and Molecular Physiology
|July 13, 2000
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Julibroside J8-induced HeLa cell apoptosis through caspase pathway.

Journal of Asian natural products research·2006
Same author

Puerarin injection for unstable angina pectoris.

The Cochrane database of systematic reviews·2006
Same author

Tuning in to the amazing outer hair cell: membrane wizardry with a twist and shout.

The Journal of membrane biology·2006
Same author

An ultra-clean firn core from the Devon Island Ice Cap, Nunavut, Canada, retrieved using a titanium drill specially designed for trace element studies.

Journal of environmental monitoring : JEM·2006
Same author

Feasibility of supercritical fluid chromatography/mass spectrometry of polypeptides with up to 40-mers.

Analytical chemistry·2006
Same author

Cortical effects of brief daily periods of unrestricted vision during early monocular form deprivation.

Journal of neurophysiology·2006

The alpha(3)-integrin subunit and its receptor, alpha(3)beta(1), are crucial for rat alveolar epithelial cell (AEC) monolayer formation. Blocking this integrin inhibits AEC adhesion and the development of confluent cell layers.

Area of Science:

  • Cell Biology
  • Integrin Signaling
  • Epithelial Cell Biology

Background:

  • Alveolar epithelial cells (AECs) form the barrier of the lung alveoli.
  • Integrins are cell surface receptors involved in cell adhesion and signaling.
  • The role of specific integrins in AEC monolayer formation requires further elucidation.

Purpose of the Study:

  • To investigate the expression of the alpha(3)-integrin subunit in rat AECs.
  • To determine the functional role of the alpha(3)-integrin subunit in AEC monolayer formation.
  • To examine the effects of blocking alpha(3)-integrin on AEC adhesion and barrier function.

Main Methods:

  • Primary culture of rat alveolar epithelial cells (AECs).
  • Detection of alpha(3)-integrin subunit mRNA and protein expression over time.

Related Experiment Videos

  • Immunoprecipitation to confirm alpha(3)- and beta(1)-integrin subunit association.
  • Treatment with anti-alpha(3)-integrin blocking monoclonal antibodies.
  • Measurement of transepithelial electrical resistance (TER).
  • Scanning electron microscopy (SEM) for monolayer morphology.

    • Main Results:

    • Alpha(3)-integrin subunit mRNA and protein were expressed in AECs and increased with culture time.
    • Alpha(3)- and beta(1)-integrin subunits formed the alpha(3)beta(1)-integrin receptor complex.
    • Blocking alpha(3)-integrin delayed and reduced TER development.
    • Anti-alpha(3) antibody treatment led to subconfluent monolayers with large gaps.
    • Alpha(3)beta(1)-integrin is essential for AEC adhesion and monolayer integrity.

    Conclusions:

    • Alpha(3)- and beta(1)-integrin subunits are expressed in AECs and form the alpha(3)beta(1)-integrin receptor.
    • The alpha(3)-integrin subunit plays a critical role in AEC adhesion.
    • Inhibition of alpha(3)-integrin function impairs the formation of confluent AEC monolayers.