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Assay for Adhesion and Agar Invasion in S. cerevisiae
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Two yeast forkhead genes regulate the cell cycle and pseudohyphal growth.

G Zhu1, P T Spellman, T Volpe

  • 1Department of Biochemistry, University of Washington, Seattle 98195-7350, USA.

Nature
|July 14, 2000
PubMed
Summary

Forkhead proteins Fkh1 and Fkh2 regulate cell cycle genes in Saccharomyces cerevisiae. Loss of these factors disrupts mitosis and M-G1 transition gene expression, impacting cell cycle progression and morphology.

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Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
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Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Saccharomyces cerevisiae exhibits cell-cycle-regulated transcription involving approximately 800 genes.
  • Some co-regulated genes form clusters, such as the 'CLB2' cluster, with genes peaking in early mitosis.

Purpose of the Study:

  • To investigate the role of forkhead transcription factors Fkh1 and Fkh2 in regulating cell cycle gene clusters.
  • To elucidate the function of Fkh proteins in the 'CLB2' and 'SIC1' gene clusters.

Main Methods:

  • Analysis of gene expression in wild-type and fkh1 fkh2 mutant Saccharomyces cerevisiae strains.
  • Chromatin immunoprecipitation to assess protein association with gene promoters.

Main Results:

  • Genes in the 'CLB2' cluster lose cell cycle regulation in the fkh1 fkh2 mutant.
  • Fkh2 protein binds to promoters of 'CLB2' cluster genes, identifying Fkh proteins as key regulators.
  • Aberrant regulation of the 'SIC1' cluster in the mutant, potentially due to altered Swi5 and Ace2 expression.
  • The fkh1 fkh2 mutant exhibits constitutive pseudohyphal morphology.

Conclusions:

  • Fkh1 and Fkh2 are essential transcription factors for the 'CLB2' gene cluster in Saccharomyces cerevisiae.
  • A transcription factor cascade involving Fkh proteins, Swi5, and Ace2 operates late in the cell cycle.
  • Fkh1 and Fkh2 play a role in controlling the switch to pseudohyphal growth.