Validation of negligible depletion solid-phase microextraction as a tool to determine tissue/blood partition...

In the current study, negligible depletion solid-phase microextraction (nd-SPME) as a technique to determine tissue/blood (tissue/water and blood/water) partition coefficients was validated. With this method the free fraction of chemicals in water in the presence of different tissues was determined and, subsequently, the partition coefficients were calculated. Liver, blood, muscle, and fat tissues obtained from male Wistar-derived rats (U:Wu) were used without homogenization. Data obtained were compared with literature data for lindane, parathion, and paraoxon. The results show that the data in the present work differ by less than a factor of two from those reported in literature. In addition, the standard deviations obtained show that the technique is accurate. Therefore, we conclude that this accurate and automated method can be used to determine tissue/blood partition coefficients for semivolatile and nonvolatile chemicals.