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The structure and expression of the Salmo salar Ran gene
M H Lundin1, B Mikkelsen, M Gudim
1Department of Morphology, Genetics and Aquatic Biology, The Norwegian School of Veterinary Science, Oslo. maria.lundin@veths.no
DNA Sequence : the Journal of DNA Sequencing and Mapping
|July 21, 2000
Summary
Researchers identified Atlantic salmon Ran genes, crucial for nuclear transport. They discovered two salmon Ran genes, Ran1 and Ran2, with conserved intron positions compared to other species.
Area of Science:
- Molecular Biology
- Genomics
- Cell Biology
Background:
- The Ran protein, a Ras-family GTPase, is vital for nucleocytoplasmic transport.
- While Ran cDNA sequences exist, no vertebrate genomic sequences were previously available.
Purpose of the Study:
- To isolate and characterize the Atlantic salmon Ran1 gene.
- To investigate the gene structure and identify different Ran transcripts in salmon.
Main Methods:
- Isolation of a genomic cosmid clone for the Atlantic salmon Ran1 gene.
- Gene structure comparison with Caenorhabditis elegans and Arabidopsis thaliana.
- Identification of Ran transcripts using molecular techniques.
Main Results:
- The Atlantic salmon Ran1 gene is approximately 2.2 kb and contains 5 introns.
- One intron was found to be conserved in position compared to C. elegans and A. thaliana.
- Two distinct Ran transcripts (Ran1 and Ran2) were identified in salmon, present in all ten tissues analyzed.
Conclusions:
- The study provides the first vertebrate genomic sequence for a Ran gene.
- The findings suggest the presence of at least two Ran genes in Atlantic salmon.
- Ran1 is expressed at higher levels than Ran2 across various salmon tissues.