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Nitric oxide increases leukocyte granule release during simulated extracorporeal circulation
M Lahtinen1, J Borowiec, P Venge
1Department of Cardiothoracic Surgery, Uppsala University Hospital, Sweden.
The Thoracic and Cardiovascular Surgeon
|July 21, 2000
Summary
Nitric oxide (NO) increased leukocyte granule proteins like myeloperoxidase (MPO) and human neutrophil lipocalin (HNL) during simulated extracorporeal circulation (SECC). However, NO did not impact oxygen free radical production in this early-stage SECC study.
Area of Science:
- Biomedical Engineering
- Immunology
- Cardiovascular Research
Background:
- Nitric Oxide (NO) is recognized for its anti-inflammatory effects.
- Leukocyte activation is a concern during extracorporeal circulation.
- Investigating NO's role in modulating leukocyte response during SECC is crucial.
Purpose of the Study:
- To determine the effect of nitric oxide (NO) on leukocyte activation during simulated extracorporeal circulation (SECC).
Main Methods:
- Human blood was subjected to a 23-hour SECC.
- The study group received NO, while the control group received a standard oxygen/air mixture.
- Leukocyte response was assessed via myeloperoxidase (MPO), human neutrophil lipocalin (HNL) release, and oxygen free radical production.
Main Results:
- NO significantly elevated MPO levels at 30 and 120 minutes and HNL levels at 120 minutes of SECC.
- Oxygen free radical production in whole blood was not significantly affected by NO.
- Chemiluminescence in isolated granulocytes showed no significant differences between groups.
Conclusions:
- Nitric oxide administration during SECC enhances the early release of leukocyte granule proteins (MPO and HNL).
- NO does not appear to influence the capacity of whole blood or isolated leukocytes to produce oxygen free radicals in this context.