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[Identification of yeast and sensitivity in vitro against different antifungal agents]
M A Muriel1, M J Vizcaíno, R Bilbao
1Departamento de Medicina Preventiva y Salud Pública, Facultad de Medicina, Universidad Autónoma de Madrid.
Introduction:
The increasing incidence of Candida yeasts infections and its hospital and community repercussion (vaginal thrush), as well as the will to acquire the knowledge of the new antifungal that were launched to the therapeutic store, have motivated us to identify that type of yeasts from different sources, as well as to study their behaviour against the antifungal, using commercial procedures with easy clinical application.
Materials And Methods:
An amount of 317 Candida yeasts were identified through commercial procedures (CHROMagar Candida and Auxacolor): 108 vaginals, 138 from ICU newborn children and 71 from ICU adults, while the antifungal drug susceptibilities was done to 199 of the isolated ones using another commercial procedure (Fungitest).
Results And Conclusions:
Candida albicans is identified as the most frequent in both hospital and community samples (78.7 and 45.93%, respectively), followed by Candida glabrata (19.44 and 28.23%, respectively). The sensitivity to amphotericin B and to 5-flucytosine was very high in every studied group, while sensitivity to imidazole derivatives depends on the samples source (lower sensitivity in the ICU newborn isolated ones) and the species (C. glabrata is less sensitive than C. albicans).
Insights
Candida albicans and Candida glabrata are the most common yeasts causing infections. Antifungal susceptibility varies by species and infection source, with amphotericin B and 5-flucytosine showing high efficacy.
Area of Science:
- Medical Mycology
- Antimicrobial Resistance
- Clinical Microbiology
Background:
- Rising incidence of Candida yeast infections in hospital and community settings.
- Need to understand the efficacy of newly available antifungal agents.
- Clinical implications of vaginal thrush and invasive candidiasis.
Purpose of the Study:
- Identify Candida yeast species from diverse clinical sources.
- Evaluate the antifungal susceptibility patterns of isolated Candida strains.
- Assess the clinical applicability of commercial identification and susceptibility testing methods.
Main Methods:
- Utilized commercial methods like CHROMagar Candida and Auxacolor for yeast identification.
- Identified 317 Candida isolates from vaginal, neonatal ICU, and adult ICU samples.
- Assessed antifungal drug susceptibility for 199 isolates using the Fungitest system.
Main Results:
- Candida albicans was the predominant species (78.7% hospital, 45.93% community).
- Candida glabrata was the second most frequent (19.44% hospital, 28.23% community).
- High sensitivity to amphotericin B and 5-flucytosine observed across all groups.
Conclusions:
- Candida albicans and Candida glabrata are key pathogens in candidiasis.
- Antifungal sensitivity to imidazole derivatives is species- and source-dependent.
- Lower sensitivity to imidazoles was noted in neonatal ICU isolates and for C. glabrata compared to C. albicans.