Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Fluorescein diffusion in the human optic disc.

I Ben-Sira, C E Riva

    Investigative Ophthalmology
    |March 1, 1975
    PubMed
    Summary
    This summary is machine-generated.

    Fluorescein dye does not diffuse across optic disc capillaries in healthy individuals. Instead, it accumulates in the optic disc tissue approximately one minute post-injection, originating from the surrounding choroid.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Compact laser Doppler choroidal flowmeter.

    Journal of biomedical optics·2012
    Same author

    [Clinic use of Laser Doppler Flowmetry (LDF) in the study of subfoveal choroidal circulation].

    La Clinica terapeutica·2011
    Same author

    Measurement by laser Doppler interferometry of intraocular distances in humans and chicks with a precision of better than ±20 µm.

    Applied optics·2010
    Same author

    Oxygen distribution in the retinal and choroidal vessels of the cat as measured by a new phosphorescence imaging method.

    Applied optics·2010
    Same author

    Near-IR retinal laser Doppler velocimetry and flowmetry: new delivery and detection techniques.

    Applied optics·2010
    Same author

    Scattering process in LDV from retinal vessels.

    Applied optics·2010
    Same journal

    Experimental aspergillosis.

    Investigative ophthalmology·2014
    Same journal

    Inhibition of prostaglandin-mediated ocular inflammatory responses by 4-biphenylacetic acid.

    Investigative ophthalmology·1976
    Same journal

    Normal interaction of plasma retinol-binding protein from retinitis pigmentosa with bovine pigment epithelium.

    Investigative ophthalmology·1976
    Same journal

    Retinol- and retinoic acid-binding proteins: occurrence in human retina and absence from human cultured fibroblasts.

    Investigative ophthalmology·1976
    Same journal

    Electrical parameters of the isolated cornea of the dogfish, Squalus acanthias.

    Investigative ophthalmology·1976
    Same journal

    Endothelial damage from intraocular lens insertion.

    Investigative ophthalmology·1976
    See all related articles

    Area of Science:

    • Ophthalmology
    • Microcirculation
    • Biomedical Optics

    Background:

    • Understanding transcapillary transfer is crucial for diagnosing optic disc pathologies.
    • Fluorescein dye is commonly used to assess ocular vasculature.
    • Previous studies have not definitively characterized fluorescein dye dynamics specifically within the optic disc.

    Purpose of the Study:

    • To investigate the characteristics of fluorescein dye transcapillary transfer in the optic disc of healthy individuals.
    • To differentiate between direct capillary leakage and diffusion from surrounding tissues into the optic disc.

    Main Methods:

    • Simultaneous measurement of fluorescein dye fluorescence and indocyanine green (ICG) absorption using a fundus reflectometer.
    • Injection of diffusible fluorescein dye and nondiffusible ICG into the systemic circulation.

    Related Experiment Videos

  • Quantification of dye concentrations in the optic disc over time.

    • Main Results:

    • Fluorescein dye was found to not diffuse across the capillaries of the optic disc.
    • Accumulation of fluorescein in the optic disc tissue began approximately one minute after injection.
    • The observed accumulation was attributed to diffusion from the adjacent choroid, with a time constant of about one minute.

    Conclusions:

    • Transcapillary transfer of fluorescein dye does not occur directly across optic disc capillaries in healthy eyes.
    • The optic disc accumulates fluorescein dye via diffusion from the choroid, not direct capillary leakage.
    • This finding has implications for interpreting fluorescein angiography in optic nerve head imaging.