Related Experiment Video
Updated: Aug 19, 2026

The Application Of Permanent Middle Cerebral Artery Ligation in the Mouse
Published on: July 25, 2011
Phospholipases A2 in ischemic and toxic brain injury
1Anesthesia and Critical Care, Massachusetts General Hospital, Charlestown 02129, USA. sapirste@helix.mgh.harvard.edu
Abstract:
Phospholipases A2 (PLA2s) regulate hydrolysis of fatty acids, including arachidonic acid, from the sn-2 position of phospholipid membranes. PLA2 activity has been implicated in neurotoxicity and neurodegenerative processes secondary to ischemia and reperfusion and other oxidative stresses. The PLA2s constitute a superfamily whose members have diverse functions and patterns of expression. A large number of PLA2s have been identified within the central nervous systems of rodents and humans. We postulated that group IV large molecular weight, cytosolic phospholipase A2 (cPLA2) has a unique role in neurotoxicity associated with ischemic or toxin stress. We created mice deficient in cPLA2 and tested this hypothesis in two injury models, ischemia/reperfusion and MPTP neurotoxicity. In each model cPLA2 deficient mice are protected against neuronal injury when compared to their wild type littermate controls. These experiments support the hypothesis that cPLA2 is an important mediator of ischemic and oxidative injuries in the brain.
More Related Videos
11:32Evaluating Cell Death Signaling by Immunofluorescence in a Rat Model of Ischemic Stroke
Published on: January 3, 2025
10:31A Liposome Membrane Permeability Assay for Investigating the Effects of Phosphatidylinositol Phosphate Groups on Membranotropic Action of Venom PLA2
Published on: September 26, 2025
Related Concept Videos
Ischemic Stroke ll: Pathophysiology
Hemorrhagic Stroke ll: Pathophysiology
Bacterial Meningitis II: Pathophysiology
Cerebral Edema ll: Pathophysiology
Cytotoxic Edema: Pathophysiology
Secondary Spinal Cord Injury llI: Pathophysiology