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Determination of reticulocytes: three methods compared.

R Siekmeier1, A Bierlich, W Jaross

  • 1Institut für Klinische Chemie und Laboratoriumsmedizin, Klinikum Carl Gustav Carus der Technischen Universität Dresden, Germany.

Clinical Chemistry and Laboratory Medicine
|July 25, 2000
PubMed
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Automated reticulocyte analysis using three different systems (ABX Pentra 120 Retic, Coulter EPICS XL MCL, and Coulter STKS) provides reliable erythropoiesis assessment. All methods show significant correlations, though minor differences in normal values should be considered.

Area of Science:

  • Hematology
  • Clinical diagnostics
  • Erythropoiesis monitoring

Background:

  • Manual reticulocyte counting is labor-intensive and lacks reproducibility.
  • Automated methods are increasingly adopted for efficient reticulocyte determination.
  • Accurate reticulocyte counts are crucial for assessing red blood cell production.

Purpose of the Study:

  • To compare the performance of three automated reticulocyte analysis systems.
  • To evaluate the reliability and reproducibility of thiazole orange and new methylene blue staining methods.
  • To assess the clinical utility of automated reticulocyte counting in routine laboratory settings.

Main Methods:

  • Analyzed 130 peripheral blood samples using ABX Pentra 120 Retic, Coulter EPICS XL MCL, and Coulter STKS.

Related Experiment Videos

  • Employed fluorescent dye thiazole orange and supravital dye new methylene blue for staining.
  • Assessed correlation, mean values, and coefficients of variation (within-batch and between-batch).

    • Main Results:

    • All three automated methods demonstrated significant correlations (r(s) ≥ 0.843, p < 0.001).
    • Coulter STKS showed slightly lower mean reticulocyte counts compared to the other two systems.
    • Within-batch coefficients of variation ranged from 6.0% to 7.8%; between-batch CVs varied by control level and method.

    Conclusions:

    • The ABX Pentra 120 Retic, Coulter EPICS XL MCL, and Coulter STKS are efficient and reliable for automated reticulocyte determination.
    • While results are similar, minor discrepancies exist, necessitating consideration for method-specific normal values.
    • Automated reticulocyte analysis offers a reproducible alternative to manual methods for erythropoiesis assessment.