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Transduction of Human Cells with Polymer-complexed Ecotropic Lentivirus for Enhanced Biosafety
Published on: July 24, 2011
Apical gene transfer into quiescent human and canine polarized intestinal epithelial cells by lentivirus vectors
J Seppen1, S C Barry, J H Klinkspoor
1Department of Pediatrics, School of Medicine, University of Washington, Washington, USA.
Journal of Virology
|July 25, 2000
Abstract:
Intestinal epithelial cells secrete a protective luminal mucus barrier inhibiting viral gene transfer. Quiescent, polarized monolayers of primary epithelial cells from dog gallbladder and human colon are efficiently transduced through the apical mucus side by lentivirus vectors, suggesting their application to intestinal gene therapy.
